Properties of Experimental and Standard Preparations of the Protective O Antigen of <I>Vibrio cholerae</I> M41 Ogawa

Abstract

Investigated are immunochemical, chemical and biochemical properties of the O-antigen preparations obtained from Vibrio cholerae M41 classical biovar, serotype Ogawa strain using standard manufacturing and improved technologies of their concentrating. The preparations have been concentrated by ultrafiltration in the mode of tangential liquid flow with membranes of various cut-off levels for a particular molar weight. ELISA has revealed equal O-antigen load in all of these preparations. Studies of specific fractures by means of chromatographic and electrophoretic methods have demonstrated their properties to be alike. Chemical composition analysis has also identified coincidence in the load of components under specification. Thus, it has been proved that application of the improved V. cholerae protective antigen concentrating technology for manufacturing cholera chemical bivalent palletized vaccine is justified.

Keywords

• vibrio cholerae
• о-антиген
• концентрирование
• ультрафильтрация
• тангенциальный режим
• иммунохимические
• химические и биохимические свойства
• o-antigen
• immuno- and biochemical properties
• concentrating
• ultrafiltration
• tangential mode