How Ocular Surface Microbiota Debuts in Type 2 Diabetes Mellitus

Frontiers in Cellular and Infection Microbiology. 2019;9 DOI 10.3389/fcimb.2019.00202

 

Journal Homepage

Journal Title: Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)

Publisher: Frontiers Media S.A.

LCC Subject Category: Science: Microbiology

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, XML

 

AUTHORS


Siqi Li (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Guoguo Yi (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Hui Peng (School of Nursing, Hunan University of Medicine, Hunan, China)

Zhenhao Li (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Shuze Chen (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Huimin Zhong (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Yifan Chen (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Zhoucheng Wang (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Qixin Deng (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

Min Fu (Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, Guangzhou, China)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 14 weeks

 

Abstract | Full Text

High glucose represents a good environment for bacterial growth on the skin, on the ocular surface (OS) and in the tears of type 2 diabetes mellitus (T2DM) patients, affecting the conjunctival bacterial community. This study aimed to investigate the OS bacterial flora of T2DM patients and healthy subjects using 16S rRNA sequencing-based bacterial identification. Among 23 healthy subjects (CON) and 31 T2DM patients, 54 eyes were examined to investigate the OS bacterial community. Factors potentially affecting the microbial growth were controlled. Results showed the OS microbiota presented higher diversity in the T2DM group than in the CON group. Bioinformatic analysis showed a lower abundance of Proteobacteria and a higher abundance of Bacteroidetes at the phyla level as well as a significantly increased abundance of Acinetobacter and Pseudomonas at the genus level in the T2DM group. The difference in OS microbiota at taxonomic level was associated with Ocular Surface Disease Index and course of T2DM. These findings indicate the OS flora in T2DM patients is significantly different from that in healthy subjects, which may be closely associated with OS discomfort and course of T2DM.