Carbon transfer from dissolved organic carbon to the cladoceran Bosmina: a mesocosm study

Abstract

A mesocosm study illuminated possible transfer pathways for dissolved organic carbon from the water column to zooplankton. Organic carbon was added as 13C enriched glucose to 15 mesocosms filled with natural lake water. Stable isotope analysis and phospholipid fatty acids-based stable isotope probing were used to trace the incorporation of 13C into the cladoceran Bosmina and its potential food items. Glucose-C was shown to be assimilated into phytoplankton (including fungi and heterotrophic protists), bacteria and Bosmina, all of which became enriched with 13C during the experiment. The study suggests that bacteria play an important role in the transfer of glucose-C to Bosmina. Furthermore, osmotic algae, fungi and heterotrophic protists might also contribute to the isotopic signature changes observed in Bosmina. These findings help to clarify the contribution of dissolved organic carbon to zooplankton and its potential pathways.

Keywords

• t-DOC
• bacteria
• Bosmina
• PLFA-SIP