Specific Combinations of Inflammatory, Angiogenesis and Vascular Integrity Biomarkers Are Associated with Clinical Severity, Coma and Mortality in Beninese Children with Plasmodium Falciparum Malaria
Bernard Tornyigah,
Samuel Odarkwei Blankson,
Rafiou Adamou,
Azizath Moussiliou,
Lauriane Rietmeyer,
Patrick Tettey,
Liliane Dikroh,
Bernard Addo,
Helena Lamptey,
Maroufou J. Alao,
Annick Amoussou,
Caroline Padounou,
Christian Roussilhon,
Sylvie Pons,
Benedicta Ayiedu Mensah,
Nicaise Tuikue Ndam,
Rachida Tahar
Affiliations
Bernard Tornyigah
Université de Paris, MERIT, IRD, 75006 Paris, France
Samuel Odarkwei Blankson
Université de Paris, MERIT, IRD, 75006 Paris, France
Rafiou Adamou
Institut de Recherche Clinique du Benin (IRCB), Calavi, Benin
Azizath Moussiliou
Institut de Recherche Clinique du Benin (IRCB), Calavi, Benin
Lauriane Rietmeyer
Université de Paris, MERIT, IRD, 75006 Paris, France
Patrick Tettey
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra P.O. Box LG 581, Ghana
Liliane Dikroh
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra P.O. Box LG 581, Ghana
Bernard Addo
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra P.O. Box LG 581, Ghana
Helena Lamptey
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra P.O. Box LG 581, Ghana
Maroufou J. Alao
Département de Pédiatrie, Hôpital Mère-Enfant la Lagune (CHUMEL), Cotonou, Benin
Annick Amoussou
Service de Pédiatrie, Centre Hospitalo-Universitaire, Suruléré (CHU-Suruléré), Cotonou, Benin
Caroline Padounou
Centre Hospitalier Universitaire de l’Oueme/Plateau, Porto-Novo, Benin
Christian Roussilhon
Unité de Génétique Fonctionnelle des Maladies Infectieuses, Département Génomes et Génétique, Institut Pasteur, 28 Rue du Docteur Roux, 75015 Paris, France
Sylvie Pons
Laboratoire Commun de Recherche Hospices Civils de Lyon-BioMérieux, Centre Hospitalier Lyon-Sud, Bâtiment 3F, 165 chemin du Grand Revoyet, 69310 Pierre-Bénite, France
Benedicta Ayiedu Mensah
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra P.O. Box LG 581, Ghana
Nicaise Tuikue Ndam
Université de Paris, MERIT, IRD, 75006 Paris, France
Rachida Tahar
Université de Paris, MERIT, IRD, 75006 Paris, France
Malaria-related deaths could be prevented if powerful diagnostic and reliable prognostic biomarkers were available to allow rapid prediction of the clinical severity allowing adequate treatment. Using quantitative ELISA, we assessed the plasma concentrations of Procalcitonin, Pentraxine-3, Ang-2, sTie-2, suPAR, sEPCR and sICAM-1 in a cohort of Beninese children with malaria to investigate their potential association with clinical manifestations of malaria. We found that all molecules showed higher levels in children with severe or cerebral malaria compared to those with uncomplicated malaria (p-value p p = 0.004 for PCT, p = 0.005 for sICAM-1, p = 0.04 for Ang-2). Moreover, except for the PCT level, the concentrations of Pentraxine-3, suPAR, sEPCR, sICAM-1, sTie-2 and Ang-2 were higher among children who died from severe malaria compared to those who survived (p = 0.037, p = 0.035, p p= 0.0008, p = 0.01 and p = 0.02, respectively). These findings indicate the ability of these molecules to accurately discriminate among clinical manifestations of malaria, thus, they might be potentially useful for the early prognostic of severe and fatal malaria, and to improve management of severe cases.
