Over-expression of transcription factor GhWRI1 in upland cotton

Abstract

Transcription factors are involved in lipid metabolism, and in present study, the transcription factor WRINKLED 1 (GhWRI1) was cloned from Gossypium hirsutum L. cv. Coker 201 by reverse transcription (RT)-PCR and rapid amplification of cDNA ends. The Pro35S:WRI1 vector was constructed and transformed into upland cotton cv. Sumian 20 using the pollen tube pathway method. After PCR and Southern blot verification of the positive transgenic plants, T2 transgenic lines derived from T1 individuals with the insertion gene in a single copy were chosen for further dissection. Semi-quantitative and quantitative RT-PCR analyses indicated that GhWRI1 gene expression increased in transgenic plants compared with that in the wild-type. Seed lipid content increased at most transgenic plants, and at the same time, protein content decreased. Compared to the control, major agronomical traits were not affected by overexpression of GhWRI1 in transgenic plants.

Keywords

• gossypium hirsutum
• transcription factor
• transgenic plants