Communications Biology (Jan 2021)
Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
Abstract
Using the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary human small airway epithelial cells. This study provides insights into LB biogenesis and membrane packing inside the LB.