Communications Biology (Jan 2021)

Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies

  • Steffen Klein,
  • Benedikt H. Wimmer,
  • Sophie L. Winter,
  • Androniki Kolovou,
  • Vibor Laketa,
  • Petr Chlanda

DOI
https://doi.org/10.1038/s42003-020-01567-z
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 12

Abstract

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Using the post-correlation on-lamella cryo-CLEM workflow, Klein, Wimmer et al. show that lamellar bodies (LBs) are composed of membrane sheets frequently attached to the limiting membrane through T-junctions in ABCA3 overexpressing cells and in primary human small airway epithelial cells. This study provides insights into LB biogenesis and membrane packing inside the LB.