Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Digvijay Singh; Samuel H. Sternberg; Jingyi Fei; Jennifer A. Doudna; Taekjip Ha

doi:10.1038/ncomms12778

Nature Communications (Sep 2016)

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Digvijay Singh,
Samuel H. Sternberg,
Jingyi Fei,
Jennifer A. Doudna,
Taekjip Ha

Affiliations

Digvijay Singh: Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
Samuel H. Sternberg: Department of Chemistry, University of California
Jingyi Fei: Department of Physics and Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign
Jennifer A. Doudna: Department of Chemistry, University of California
Taekjip Ha: Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

DOI: https://doi.org/10.1038/ncomms12778
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 8

Abstract

Read online

CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal