Lignocellulosic xylitol production from corncob using engineered Kluyveromycesmarxianus

Jia Zhang; Jia Zhang; Jia Zhang; Jia Zhang; Teng Xu; Teng Xu; Xiaohang Wang; Xiaohang Wang; Xiaohang Wang; Xiaoyan Jing; Xiaoyan Jing; Xiaoyan Jing; Jia Zhang; Jiong Hong; Jiong Hong; Jian Xu; Jian Xu; Jian Xu; Jichao Wang

doi:10.3389/fbioe.2022.1029203

Frontiers in Bioengineering and Biotechnology (Oct 2022)

Lignocellulosic xylitol production from corncob using engineered Kluyveromycesmarxianus

Jia Zhang,
Jia Zhang,
Jia Zhang,
Jia Zhang,
Teng Xu,
Teng Xu,
Xiaohang Wang,
Xiaohang Wang,
Xiaohang Wang,
Xiaoyan Jing,
Xiaoyan Jing,
Xiaoyan Jing,
Jia Zhang,
Jiong Hong,
Jiong Hong,
Jian Xu,
Jian Xu,
Jian Xu,
Jichao Wang

Affiliations

Jia Zhang: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
Jia Zhang: Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Jia Zhang: University of Chinese Academy of Sciences, Beijing, China
Jia Zhang: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China
Teng Xu: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
Teng Xu: University of Chinese Academy of Sciences, Beijing, China
Xiaohang Wang: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
Xiaohang Wang: Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Xiaohang Wang: University of Chinese Academy of Sciences, Beijing, China
Xiaoyan Jing: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
Xiaoyan Jing: Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Xiaoyan Jing: University of Chinese Academy of Sciences, Beijing, China
Jia Zhang: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China
Jiong Hong: School of Life Sciences, University of Science and Technology of China, Hefei, China
Jiong Hong: Hefei National Laboratory for Physical Science at the Microscale, Hefei, China
Jian Xu: Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China
Jian Xu: Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Jian Xu: University of Chinese Academy of Sciences, Beijing, China
Jichao Wang: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China

DOI: https://doi.org/10.3389/fbioe.2022.1029203
Journal volume & issue: Vol. 10

Abstract

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Xylitol production from lignocellulose hydrolysate is a sustainable and environment-friendly process. In this study, a systematic process of converting corncob waste into xylitol is described. First, the corncobs are hydrolyzed with acid to a hydrolysate. Second, Kluyveromyces marxianus YZJQ016 derived from K. marxianus YZJ074, constructed by overexpressing ScGAL2-N376F from Saccharomyces cerevisiae, CtXYL1 from Candida tropicalis, and KmZWF1 from K. marxianus, produces xylitol from the hydrolysate. A total of ten xylose reductase genes were evaluated, and CtXYL1 proved best by showing the highest catalytic activity under the control of the KmGAPDH promoter. A 5 L fermenter at 42°C produced 105.22 g/L xylitol using K. marxianus YZJQ016—the highest production reported to date from corncob hydrolysate. Finally, for crystallization of the xylitol, the best conditions were 50% (v/v) methanol as an antisolvent, at 25°C, with purity and yield of 99%–100% and 74%, respectively—the highest yield reported to date.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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