Iraqi Journal of Hematology (Jan 2016)
Inducing of Interleukins-10 and 8 by Epstein Barr Virus in Chronic Lymphocytic Leukemia
Abstract
Background : Many newer studies reported that Epstein- Barr virus (EBV) has association with chronic lymphocytic leukemia (CLL). The average age of presentation is of patients with CLL is between 65 and 70 years with male to female ratio is 2:1. Notably, several studies have reported that expression of Epstein Barr encoding RNA (EBERs) is associated with progressive or accelerated clinical courses. This type of RNA increases the level of IL-8 and IL-10 in serum of newly diagnosis of CLL patients. Objectives: the aims of study were to determine the interleukins 10 and 8 level in newly diagnosed CLL patients and determine the incidence of EBV infection in patient of CLL. Materials and Methods: A prospective study conducted at department of clinical hematology in the national center of hematology in Baghdad, Iraq from January 2013 to January 2014. It included thirty samples of formalin-fixed, paraffin-embedded tissue of bone marrow aspirates samples and blood from newly diagnosis B-CLL. They were diagnosed with CLL according to Binet criteria. The detection of EBV encoded RNAs (EBER1, EBER2) and also detection for the level of Interleukin (8 and 10) in the serum of CLL patients were done by in situ hybridization technique. Results: Histopathological study revealed that all the controls were negative for EBERs and 46.7% were found to be EBERs positive. There was a correlation between positive EBERs and tumors stage and also EBERs and IL-10 and with IL-8. Conclusion: The highest incidence of CLL occurs in the age group 40-80 years old and males are more liable than females (male/female: 70/30).In situ hybridization technique is successful method to detect of EBV and positive EBERs. IL-8 is highly significant in CLL patients and correlates with EBERs and LMP1. There is a correlation between IL-10 EBERs and LMP1 in CLL patients.
