Clinical Interventions in Aging (Mar 2020)
Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study
Abstract
Mojgan Sheikhpour,1,2 Darioush Eliaspour,3 Iraj Arabi,3 Seyed Ahmad Raeissadat,3 Arezou Lari,4 Tohid Seif Barghi5 1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; 3Shahid Beheshti University of Medical Sciences, Department of Physical Medicine and Rehabilitation, Tehran, Iran; 4Biomedicine Unit, Pasteur Institute of Iran, Tehran, Iran; 5Department of Sports and Exercise Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, IranCorrespondence: Mojgan Sheikhpour; Darioush Eliaspour Tel +98 9122969712; +98 9125707896Fax +98 21 64112313Email [email protected]; [email protected]: Osteoarthritis is the most common type of arthritis and one of the leading causes of job loss and motor disabilities. Recently, the involvement of dopaminergic pathways and dopamine receptor genes has been considered in this disease. Therefore, studying and comparing the expression pattern of these receptor genes can lead to a greater understanding of the pathogenesis of this disease.Methods: In this research, we used the systems biology approach to investigate the role of the dopaminergic pathway in osteoarthritis. Then the gene expression pattern of dopamine receptor genes was examined in an osteoarthritis patientgroup in comparison with healthy individuals by Real-time PCR method.Results: The analysis of the transcriptome dataset of osteoarthritis identified some genes in the dopaminergic pathway and the six most important genes in this disease are in the network with a significant relationship to dopamine receptors which differentially expressed compared to health groups. Statistical analysis of the case control study showed a significant difference (P-value< 0.05) in DRD1 and DRD2 family in the patients in comparison to healthy individuals.Discussion: We attained the significant expression pattern of dopamine receptors in the blood of osteoarthritis patients which could be useful to identify new strategies for the diagnosis, management, or treatment of this disease.Keywords: osteoarthritis, systems biology, dopamine receptor genes, gene expression
