Identification of stably expressed QTL for resistance to black shank disease in tobacco (Nicotiana tabacum L.) line Beinhart 1000-1
Yusheng Zhang,
Xuan Guo,
Xingxing Yan,
Min Ren,
Caihong Jiang,
Yazeng Cheng,
Liuying Wen,
Dan Liu,
Yu Zhang,
Mingming Sun,
Quanfu Feng,
Aiguo Yang,
Lirui Cheng
Affiliations
Yusheng Zhang
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Xuan Guo
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China; College of Plant Science and Technology, Qingdao Agricultural University, Qingdao 266109, Shandong, China
Xingxing Yan
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Min Ren
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Caihong Jiang
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Yazeng Cheng
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Liuying Wen
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Dan Liu
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Yu Zhang
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Mingming Sun
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Quanfu Feng
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China
Aiguo Yang
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China; Corresponding author.
Lirui Cheng
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong, China; Corresponding author.
Cigar line Beinhart 1000-1 has effective durable resistance to black shank (BS) and is considered one of the most resistant sources in tobacco (Nicotiana tabacum L.). To investigate the inheritance and identification of stable quantitative trait loci (QTL) for BS response, F2, BC1F2 individuals and BC1F2:3 lines were produced from a cross between Beinhart 1000-1 and Xiaohuangjin 1025. Two major quantitative trait loci (M-QTL) named qBS7 and qBS17 were repeatedly detected under different conditions. QTL qBS7 was mapped to the region between PT30174 and PT60621 and explained 17.40%–25.60% of the phenotypic variance under different conditions. The other QTL qBS17 in interval PT61564–PT61538 of linkage group 17 was detected in a BC1F2 population in the field and in BC1F2:3 in both the field and at the seedling stage, explaining 6.90% to 11.60% of the phenotypic variance. The results improve our understanding of the inheritance of resistance to BS and provide information that can be used in marker-assisted breeding. Keywords: Phytophthora nicotianae, Quantitative trait locus, Plant disease resistance, Simple sequence repeats
