Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption

Changchang Xin; Jianhang Yin; Shaopeng Yuan; Liqiong Ou; Mengzhu Liu; Weiwei Zhang; Jiazhi Hu

doi:10.1038/s41467-022-33346-1

Nature Communications (Sep 2022)

Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption

Changchang Xin,
Jianhang Yin,
Shaopeng Yuan,
Liqiong Ou,
Mengzhu Liu,
Weiwei Zhang,
Jiazhi Hu

Affiliations

Changchang Xin: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Jianhang Yin: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Shaopeng Yuan: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Liqiong Ou: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Mengzhu Liu: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Weiwei Zhang: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University
Jiazhi Hu: The MOE Key Laboratory of Cell Proliferation and Differentiation, Genome Editing Research Center, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University

DOI: https://doi.org/10.1038/s41467-022-33346-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

Read online

CRISPR-Cas12f nucleases can be effectively packaged into AAVs for gene therapy, but a systematic evaluation of editing outcomes is lacking. Here the authors perform a comprehensive assessment of 4 Cas12f proteins and compare to Cas9 and two Cas12a proteins at a number of sites.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal