FRET-FISH probes chromatin compaction at individual genomic loci in single cells

Ana Mota; Szymon Berezicki; Erik Wernersson; Luuk Harbers; Xiaoze Li-Wang; Katarina Gradin; Christiane Peuckert; Nicola Crosetto; Magda Bienko

doi:10.1038/s41467-022-34183-y

Nature Communications (Nov 2022)

FRET-FISH probes chromatin compaction at individual genomic loci in single cells

Ana Mota,
Szymon Berezicki,
Erik Wernersson,
Luuk Harbers,
Xiaoze Li-Wang,
Katarina Gradin,
Christiane Peuckert,
Nicola Crosetto,
Magda Bienko

Affiliations

Ana Mota: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Szymon Berezicki: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Erik Wernersson: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Luuk Harbers: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Xiaoze Li-Wang: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Katarina Gradin: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Christiane Peuckert: Stockholm University, The Department of Molecular Biosciences, The Wenner-Gren Institute
Nicola Crosetto: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Magda Bienko: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

DOI: https://doi.org/10.1038/s41467-022-34183-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Chromatin compaction affects many nuclear processes yet compaction levels at individual genomic loci have been notoriously difficult to assess. Here, Ana Mota and co-authors from the Bienko-Crosetto Lab present FRET-FISH for probing chromatin compaction at selected loci in single cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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