Effect of mesenchymal stem cells cultivation with erythropoietin on their morphofunctional properties

Abstract

Mesenchymal stem cells (MSCs) are considered as a promising tool for the treatment of inflammatory and degenerative diseases, but their effectiveness is associated with the survival of cells in an unfavorable microenvironment. Erythropoietin exhibits an anti-apoptotic effect for non-hematopoietic cells of the human and animal body. The aim of the study was to evaluate the morphofunctional properties of MSCs cultured with erythropoietin. Material and methods. MSCs were obtained from bone marrow aspirate of patients with chronic heart failure. The primary culture of MSCs-EPO was obtained by growth MSCs of the 4th passage for 7-8 days with erythropoietin at a dose of 33 IU/mL. The phenotype, differentiation in the adipogenic and osteogenic directions, proliferation, migration, and production of nitric oxide of MSCs were studied. Results and discussion. The study revealed that the cultivation of MSCs with erythropoietin contributes to an increase in the expression of the main surface molecules that characterize the cells belonging to true MSCs, possess to differentiated of MSCs into adipocytes and osteoblasts, a change in the expression levels of adhesion molecules, a decrease in the level of apoptosis, increased cell migration during the induction of oxidative stress and hyperglycemia. Conclusion. Cultivation of MSCs with erythropoietin increases the resistance of cells to adverse microenvironment factors.

Keywords

• bone marrow mesenchymal stem cells
• erythropoietin
• proliferation
• colony formation
• migration
• adhesion molecules
• cell cycle
• apoptosis/necrosis
• nitric oxide