DNA EXTRACTION PROTOCOL FOR USE IN PRACTICAL LESSONS IN HIGHER EDUCATION

Abstract

Increasingly the biotechnology is important in everyday life, in the most varied areas. The discovery of DNA and its manipulation has brought a world of possibilities to modern society, and the proper training of professionals in this area is a crucial point. The way in which information is passed, the visualization of what is being learned, through practical classes, are determining factors in the teaching-learning process. Considering the weight of practical classes to train more qualified professionals and the limitation faced by professors and institutions to offer such practices, a DNA extraction protocol was developed to use in practical class with basis in team knowhow. The extraction protocol was developed to be more efficient, using regents easier to be acquired and manipulated, being initially tested with biological yeast. The protocol showed efficient to yeast DNA extraction and was also tested with pure bacterial culture. This protocol extracted genetic material with quantity and quality enough to amplification, it showed be a simple, cheaper and efficient protocol for practical class use.