Frontiers in Cellular and Infection Microbiology (Jul 2021)
MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line
- Jun-Hong Wang,
- Jun-Hong Wang,
- Jun-Hong Wang,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Yi-Yuan Lu,
- Yi-Yuan Lu,
- Yi-Yuan Lu,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Ming-Yang Cheng,
- Ming-Yang Cheng,
- Ming-Yang Cheng,
- Ru-Yu Wang,
- Ru-Yu Wang,
- Ru-Yu Wang,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Wen-Tao Yang,
- Wen-Tao Yang,
- Wen-Tao Yang,
- Dan-Dan Zhao,
- Dan-Dan Zhao,
- Dan-Dan Zhao,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Li-Ping Ye,
- Li-Ping Ye,
- Li-Ping Ye,
- Xin Cao,
- Xin Cao,
- Xin Cao,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Chun-Feng Wang,
- Chun-Feng Wang,
- Chun-Feng Wang
Affiliations
- Jun-Hong Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Jun-Hong Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Jun-Hong Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yi-Yuan Lu
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yi-Yuan Lu
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yi-Yuan Lu
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan Zeng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Ming-Yang Cheng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ming-Yang Cheng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Ming-Yang Cheng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Ru-Yu Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ru-Yu Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Ru-Yu Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yu Sun
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yu Sun
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yu Sun
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Dan-Dan Zhao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Dan-Dan Zhao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Dan-Dan Zhao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Li-Ping Ye
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Li-Ping Ye
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Li-Ping Ye
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Cao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xin Cao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fcimb.2021.706919
- Journal volume & issue
-
Vol. 11
Abstract
Zinc finger and BTB domain containing 1(Zbtb1) is a transcriptional suppressor protein, and a member of the mammalian Zbtb gene family. Previous studies have shown that Zbtb1 is essential for T-cell development. However, the role of Zbtb1 in T-cell lymphoma is undetermined. In this study, an EL4 cell line with Zbtb1 deletion was constructed using the CRISPR-Cas9 technique. The expression profiles of microRNA and circRNA produced by the control and gene deletion groups were determined by RNA-seq. In general, 24 differentially expressed microRNA and 16 differentially expressed circRNA were found between normal group and gene deletion group. Through further analysis of differentially expressed genes, GO term histogram and KEGG scatter plot were drawn, and three pairs of miRNA and circRNA regulatory relationships were found. This study describes the differentially expressed microRNA and circRNA in normal and Zbtb1-deficient EL4 cell lines, thus providing potential targets for drug development and clinical treatment of T-cell lymphoma.
Keywords
