Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats
Karina Nascimento da Silva,
Aline Garnevi Fávero,
William Ribeiro,
Caroline Marcantonio Ferreira,
Patrícia Sartorelli,
Leonardo Cardili,
Cristina Stewart Bogsan,
Joice Naiara Bertaglia Pereira,
Rita de Cássia Sinigaglia,
Andréa Cristina de Moraes Malinverni,
Ana Paula Ribeiro Paiotti,
Sender Jankiel Miszputen,
Orlando Ambrogini-Júnior
Affiliations
Karina Nascimento da Silva
Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil
Aline Garnevi Fávero
Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil
William Ribeiro
Institute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, Brazil
Caroline Marcantonio Ferreira
Institute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, Brazil
Patrícia Sartorelli
Institute of Environmental, Chemistry and Pharmaceutical Sciences, Department of Pharmaceutics Sciences - Universidade Federal de São Paulo, Diadema, SP, Brazil
Leonardo Cardili
Laboratory of Experimental and Molecular Pathology, Department of Pathology - Universidade Federal de São Paulo, São Paulo, SP, Brazil
Cristina Stewart Bogsan
Laboratory of Fermented Foods of the Faculty of Pharmaceutical Sciences – University of São Paulo
Joice Naiara Bertaglia Pereira
Butantan Institute, São Paulo, SP, Brazil
Rita de Cássia Sinigaglia
Electron Microscopy Centre – Universidade Federal de São Paulo, São Paulo, SP, Brazil
Andréa Cristina de Moraes Malinverni
Laboratory of Experimental and Molecular Pathology, Department of Pathology - Universidade Federal de São Paulo, São Paulo, SP, Brazil
Ana Paula Ribeiro Paiotti
Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil; Corresponding author.
Sender Jankiel Miszputen
Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil
Orlando Ambrogini-Júnior
Division of Gastroenterology, Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP, SP, Brazil
Background and aim: The etiopathogenesis of inflammatory bowel disease (IBD) is associated with different factors such as genetic, infectious, immunological, and environmental, including modification of the gut microbiota. IBD′s conventional pharmacological therapeutic approaches have become a challenge due to side effects, complications from prolonged use, and higher costs. Kefir fermented milk beverage is a functional food that has demonstrated multiple beneficial effects including anti-inflammatory and antioxidant activity. Alternative therapeutic strategies have been used for IBD as more natural products with low-cost and easy acquisition. The aim of this study is to evaluate the anti-inflammatory effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats. Methods: We used 4 groups to perform this study: baseline control (BC), kefir control (KC), 5% untreated DSS-induced colitis (DSS), and 5% DSS-induced colitis treated with kefir (DSSK). The animals received fermented kefir milk beverage ad libitum for six days and the disease activity index was recorded daily. Colon samples were processed for Transmission Electron Microscopy and histopathological evaluation. We analyzed short fatty chain acids through the fecal sample using gas chromatography. Results: Kefir supplementation was able to reduce the clinical activity index and inflammatory process evidenced by decreased neutrophil accumulation, decreased reticulum edema, and increased autophagosomes. Also, showed a trend to increase the levels of acetate and propionate. Conclusions: Our results suggest that kefir fermented milk beverage may have an anti-inflammatory effect minimizing the intestinal damage of DSS-induced colitis.
