High-precision estimation of emitter positions using Bayesian grouping of localizations

Mohamadreza Fazel; Michael J. Wester; David J. Schodt; Sebastian Restrepo Cruz; Sebastian Strauss; Florian Schueder; Thomas Schlichthaerle; Jennifer M. Gillette; Diane S. Lidke; Bernd Rieger; Ralf Jungmann; Keith A. Lidke

doi:10.1038/s41467-022-34894-2

Nature Communications (Nov 2022)

High-precision estimation of emitter positions using Bayesian grouping of localizations

Mohamadreza Fazel,
Michael J. Wester,
David J. Schodt,
Sebastian Restrepo Cruz,
Sebastian Strauss,
Florian Schueder,
Thomas Schlichthaerle,
Jennifer M. Gillette,
Diane S. Lidke,
Bernd Rieger,
Ralf Jungmann,
Keith A. Lidke

Affiliations

Mohamadreza Fazel: Department of Physics and Astronomy, University of New Mexico
Michael J. Wester: Department of Physics and Astronomy, University of New Mexico
David J. Schodt: Department of Physics and Astronomy, University of New Mexico
Sebastian Restrepo Cruz: Department of Pathology, University of New Mexico Health Science Center
Sebastian Strauss: Department of Physics and Center for Nanoscience, Ludwig Maximilian University
Florian Schueder: Department of Physics and Center for Nanoscience, Ludwig Maximilian University
Thomas Schlichthaerle: Department of Physics and Center for Nanoscience, Ludwig Maximilian University
Jennifer M. Gillette: Department of Pathology, University of New Mexico Health Science Center
Diane S. Lidke: Department of Pathology, University of New Mexico Health Science Center
Bernd Rieger: Department of Imaging Physics, Delft University of Technology
Ralf Jungmann: Department of Physics and Center for Nanoscience, Ludwig Maximilian University
Keith A. Lidke: Department of Physics and Astronomy, University of New Mexico

DOI: https://doi.org/10.1038/s41467-022-34894-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

Single-molecule localization microscopy relies on stochastic blinking events, treated as independent events without assignment to a particular emitter. Here, BaGoL takes low precision localizations generated from multiple emitter blinkings during DNAPAINT and dSTORM and finds the underlying emitter positions with high precision.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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