International Journal of Infectious Diseases (Jul 2022)

Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

  • Meng Yee Lai,
  • Fatma Diyana Mohd Bukhari,
  • Nur Zulaikha Zulkefli,
  • Ilyiana Ismail,
  • Nur Izati Mustapa,
  • Tuan Suhaila Tuan Soh,
  • Afifah Haji Hassan,
  • Kalaiarasu M Peariasamy,
  • Yee Leng Lee,
  • Jeyanthi Suppiah,
  • Ravindran Thayan,
  • Yee Ling Lau

Journal volume & issue
Vol. 120
pp. 132 – 134

Abstract

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ABSTRACT: Objectives: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix. Methods: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method. Results: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78–98.2%) and 100% specificity (95% confidence interval: 92–100%) using UDG RT-LAMP. Conclusion: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.

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