Dipartimento di Fisica e Astronomia “Ettore Majorana”, Università degli Studi di Catania, Via S. Sofia 64, 95123 Catania, Italy
Dario Morganti
Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche, ed Ambientali, Università degli Studi di Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, Italy
Angelina Midiri
Dipartimento di Patologia Umana, Università di Messina, Via Consolare Valeria 1, (Azienda Ospedaliera Universitaria Policlinico “G. Martino”), 98125 Messina, Italy
The continuing accumulation of mutations in the RNA genome of the SARS-CoV-2 virus generates an endless succession of highly contagious variants that cause concern around the world due to their antibody resistance and the failure of current diagnostic techniques to detect them in a timely manner. Raman spectroscopy represents a promising alternative to variants detection and recognition techniques, thanks to its ability to provide a characteristic spectral fingerprint of the biological samples examined under all circumstances. In this work we exploit the surface-enhanced Raman scattering (SERS) properties of a silver dendrite layer to explore, for the first time to our knowledge, the distinctive features of the Omicron variant genome. We obtain a complex spectral signal of the Omicron variant genome where the fingerprints of nucleobases in nucleosides are clearly unveiled and assigned in detail. Furthermore, the fractal SERS layer offers the presence of confined spatial regions in which the analyte remains trapped under hydration conditions. This opens up the prospects for a prompt spectral identification of the genome in its physiological habitat and for a study on its activity and variability.
