Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia
Larisa Suturina,
Daria Lizneva,
Alina Atalyan,
Ludmila Lazareva,
Aleksey Belskikh,
Tatyana Bairova,
Leonid Sholokhov,
Maria Rashidova,
Irina Danusevich,
Iana Nadeliaeva,
Lilia Belenkaya,
Zorikto Darzhaev,
Eldar Sharifulin,
Natalia Belkova,
Ilia Igumnov,
Tatyana Trofimova,
Anastasiya Khomyakova,
Kseniia Ievleva,
Natalia Babaeva,
Irina Egorova,
Madinabonu Salimova,
Bulent O. Yildiz,
Richard S. Legro,
Frank Z. Stanczyk,
Ricardo Azziz
Affiliations
Larisa Suturina
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Daria Lizneva
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Alina Atalyan
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Ludmila Lazareva
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Aleksey Belskikh
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Tatyana Bairova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Leonid Sholokhov
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Maria Rashidova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Irina Danusevich
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Iana Nadeliaeva
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Lilia Belenkaya
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Zorikto Darzhaev
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Eldar Sharifulin
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Natalia Belkova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Ilia Igumnov
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Tatyana Trofimova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Anastasiya Khomyakova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Kseniia Ievleva
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Natalia Babaeva
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Irina Egorova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Madinabonu Salimova
Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, Russia
Bulent O. Yildiz
Division of Endocrinology and Metabolism, Hacettepe University School of Medicine, Hacettepe, 06100 Ankara, Turkey
Richard S. Legro
Hershey Medical Center, Penn State College of Medicine, 500 University Dr, Hershey, PA 17033, USA
Frank Z. Stanczyk
Keck School of Medicine, University of Southern California, 1975 Zonal Ave, Los Angeles, CA 90033, USA
Ricardo Azziz
School of Medicine and Public Health, University of Alabama at Birmingham, 1700 6th Ave, South Birmingham, AL 35249, USA
Androgen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy control” women, overall and by ethnicity (Caucasian and Asian), in the cross-sectional Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (ClinicalTrials.gov ID: NCT05194384) conducted in 2016–2019. Overall, we identified a “healthy control” group consisting of 143 healthy premenopausal women without menstrual dysfunction, hirsutism, polycystic ovaries, or medical disorders. We analyzed serum total testosterone (TT) by using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and DHEAS, sex-hormone-binding globulin (SHBG), TSH, prolactin, and 17-hydroxyprogesterone (17OHP) were assessed with an enzyme-linked immunosorbent assay (ELISA). The UNLs for the entire population for the TT, free androgen index (FAI), and DHEAS were determined as the 98th percentiles in healthy controls as follows: 67.3 (95% confidence interval (CI): 48.1, 76.5) ng/dl, 5.4 (3.5, 14.0), and 355 (289, 371) μg/dl, respectively. The study results demonstrated that the UNLs for TT and FAI varied by ethnicity, whereas the DHEAS UNLs were comparable in the ethnicities studied.
