Frontiers in Microbiology (Sep 2019)

Metagenomic Investigation of Torque Teno Mini Virus-SH in Hematological Patients

  • Antonin Bal,
  • Antonin Bal,
  • Antonin Bal,
  • Guy Oriol,
  • Laurence Josset,
  • Laurence Josset,
  • Laurence Generenaz,
  • Clémentine Sarkozy,
  • Pierre Sesques,
  • Gilles Salles,
  • Florence Morfin,
  • Florence Morfin,
  • Bruno Lina,
  • Bruno Lina,
  • Jérémie Becker,
  • Frédéric Reynier,
  • François Mallet,
  • Alexandre Pachot,
  • Valérie Cheynet,
  • Karen Brengel-Pesce,
  • Sophie Trouillet-Assant,
  • Sophie Trouillet-Assant

DOI
https://doi.org/10.3389/fmicb.2019.01898
Journal volume & issue
Vol. 10

Abstract

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A new member of Anelloviridae, named torque teno mini virus (TTMV)-SH, was recently identified in the serum of three Hodgkin’s lymphoma patients suggesting that TTMV-SH may be associated with this type of hematological malignancy. We investigated by metagenomic analysis the presence of TTMV-SH-related viruses in plasma samples (n = 323) collected from patients with various hematological malignancies (multiple myeloma (MM, n = 256), non-Hodgkin’s lymphoma (NHL, n = 20), acute myeloid leukemia (n = 10)) and from healthy donors (n = 37). TTMV-SH-related strains were identified in 24 samples corresponding to four MM and one NHL patients. Phylogenic analysis revealed that the 24 isolates were close to the TTMV-SH strains previously identified, sharing 79.6–86.7% ORF1 nucleotide sequence identity. These results suggest that TTMV-SH-related viruses might be found in hematological diseases other than Hodgkin’s lymphoma. Due to the high genetic variability within Anelloviridae species, the association between a particular medical condition and a new genotype should be interpreted with caution.

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