Physicochemical and Biological Study of <sup>99m</sup>Tc and <sup>68</sup>Ga Radiolabelled Ciprofloxacin and Evaluation of [<sup>99m</sup>Tc]Tc-CIP as Potential Diagnostic Radiopharmaceutical for Diabetic Foot Syndrome Imaging
Przemysław Koźmiński,
Weronika Gawęda,
Magdalena Rzewuska,
Agata Kopatys,
Szymon Kujda,
Marta K. Dudek,
Paweł Krzysztof Halik,
Leszek Królicki,
Ewa Gniazdowska
Affiliations
Przemysław Koźmiński
Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland
Weronika Gawęda
Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland
Magdalena Rzewuska
Institute of Veterinary Medicine, Department of Preclinical Sciences, Division of Microbiology, Warsaw University of Life Sciences—SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland
Agata Kopatys
Department of Nuclear Medicine, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland
Szymon Kujda
Department of Nuclear Medicine, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland
Marta K. Dudek
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland
Paweł Krzysztof Halik
Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland
Leszek Królicki
Department of Nuclear Medicine, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland
Ewa Gniazdowska
Centre of Radiochemistry and Nuclear Chemistry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland
This paper presents the application of ciprofloxacin as a biologically active molecule (vector) for delivering diagnostic radiopharmaceuticals to the sites of bacterial infection. Ciprofloxacin-based radioconjugates containing technetium-99m or gallium-68 radionuclides were synthesised, and their physicochemical (stability, lipophilicity) and biological (binding study to Staphylococcus aureus and Pseudomonas aeruginosa) properties were investigated. Both the tested radiopreparations met the requirements for radiopharmaceuticals, and technetium-99m-labelled ciprofloxacin turned out to be a good radiotracer for the tomography of diabetic foot syndrome using SPECT.
