Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Yujie Hua; Yingying Huo; Baoshuai Bai; Junxiang Hao; Guanhuai Hu; Zheng Ci; Xiaodi Wu; Mengyuan Yu; Xin Wang; Hong Chen; Wenjie Ren; Yixin Zhang; Xiaoyun Wang; Guangdong Zhou

Materials Today Bio (Dec 2022)

Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Yujie Hua,
Yingying Huo,
Baoshuai Bai,
Junxiang Hao,
Guanhuai Hu,
Zheng Ci,
Xiaodi Wu,
Mengyuan Yu,
Xin Wang,
Hong Chen,
Wenjie Ren,
Yixin Zhang,
Xiaoyun Wang,
Guangdong Zhou

Affiliations

Yujie Hua: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China; National Tissue Engineering Center of China, Shanghai, PR China; Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China
Yingying Huo: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China; National Tissue Engineering Center of China, Shanghai, PR China
Baoshuai Bai: Department of Orthopaedics, Qilu Hospital of Shangdong University Centre for Orthopaedics, Advanced Medical Research Institute, Shangdong University, Shangdong, PR China
Junxiang Hao: Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China; National Tissue Engineering Center of China, Shanghai, PR China
Guanhuai Hu: Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China
Zheng Ci: Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China; National Tissue Engineering Center of China, Shanghai, PR China
Xiaodi Wu: Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China; National Tissue Engineering Center of China, Shanghai, PR China
Mengyuan Yu: Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China
Xin Wang: Department of Hand Surgery, Ningbo Sixth Hospital, Zhejiang, PR China
Hong Chen: Department of Hand Surgery, Ningbo Sixth Hospital, Zhejiang, PR China
Wenjie Ren: Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China; Corresponding author.
Yixin Zhang: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China
Xiaoyun Wang: Department of Plastic Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China; Corresponding author.
Guangdong Zhou: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China; Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China; National Tissue Engineering Center of China, Shanghai, PR China; Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China; Corresponding author. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.

Journal volume & issue: Vol. 17
p. 100489

Abstract

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The fabrication of biphasic cartilage-bone integrated scaffolds is an attractive alternative for osteochondral repair but has proven to be extremely challenging. Existing three-dimensional (3D) scaffolds are insufficient to accurately biomimic the biphasic cartilage-bone integrated microenvironment. Currently, photo-crosslinkable hydrogels based on tissue-specific decellularized extracellular matrix (dECM) have been considered as an important technique to fabricate biomimetic scaffolds, but so far there has been no breakthrough in the photo-crosslinkable hydrogel scaffolds with biphasic cartilage-bone biomimetic microenvironment. Here, we report a novel strategy for the preparation of biomimetic cartilage-bone integrated scaffolds based on photo-crosslinkable cartilage/bone-derived dECM hydrogels, which are able to reconstruct biphasic cartilage-bone biomimetic microenvironment. The biphasic cartilage-bone integrated scaffolds provided a 3D microenvironment for osteochondral regeneration. The cartilage biomimetic scaffolds, consisting of cartilage-derived dECM hydrogels, efficiently regulated chondrogenesis of bone marrow mesenchymal stem cells (BMSCs). The bone biomimetic scaffolds, composed of cartilage/bone-derived dECM hydrogels, first regulated chondrogenesis of BMSCs, followed by endochondral ossification over time. Taken together, the biphasic cartilage-bone integrated tissue could be successfully reconstructed by subcutaneous culture based on cartilage-bone bilayered structural design. Furthermore, the biphasic cartilage-bone biomimetic scaffolds (cell-free) achieved satisfactory cartilage-bone integrated regeneration in the osteochondral defects of rabbits’ knee joints.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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