Characteristics of Pollen from Transgenic Lines of Apple Carrying the Exogenous CpTI Gene

Abstract

It is fundamental for gene transformation and ecosystem hazard evaluation to study the pollen characteristics of transgenic plants. In this research, the characteristics of pollen from 7- or 8-year-old transgenic apple plants carrying an exogenous CpTI gene were analyzed. The results showed that there was no significant difference in terms of size, morphology, or exine ornamentation between the pollen of the transgenic plants and the non-transgenic control. However, the transgenic plants had more abnormal pollen grains. Of the 13 transgenic lines tested, 12 had a significantly lower amount of pollen and six exhibited a significantly lower germination rate when cultured in vitro. The pollen viability of three transgenic lines was determined, with two showing significantly lower viability than the control. The transgenic Gala apple pollen grains germinated normally via controlled pollination on Fuji apple stigmas. However, the pollen tubes extended relatively slowly during the middle and late development stages, and another 8 h were needed to reach the ovules compared with the control. The gibberellic acid concentration in transgenic Gala apple flowers was lower than in the non-transgenic control during all development stages tested. The abscisic acid concentration in the transgenic flowers was lower during the pink stage, and higher during the ball and fully open stages. Microscopic observation of the anther structure showed no difference. The tapetum of the pollen sac wall in transgenic plants decomposed late and affected pollen grain development, which could be one of the reasons for the lower number of pollen grains and poor viability in the transgenic plants.

Keywords

• apple
• exogenous gene
• pollen
• viability
• tapetum