Journal of Pharmacopuncture (Sep 2013)

Differential Expression of Gene Profiles in MRGX-treated Lung Cancer

  • Kwon Yong-Kyun ,
  • Lee Seung-Yeul ,
  • Kang Hwan-Soo ,
  • Sung Jung-Suk ,
  • Cho Chong-Kwan ,
  • Yoo Hwa-Seung ,
  • Shin Seungjin ,
  • Choi Jong-Soon ,
  • Lee Yeon-Weol ,
  • Jang Ik-Soon

DOI
https://doi.org/10.3831/KPI.2013.16.018
Journal volume & issue
Vol. 16, no. 3
pp. 30 – 38

Abstract

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Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.

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