Analysis of Common Herbicides in Blood by UPLC-HRMS

YANG Yang, ZHANG Xiao-guang, YU Feng，et al .

doi:10.12116/j.issn.1004-5619.2018.06.003

Fayixue Zazhi (Dec 2018)

Analysis of Common Herbicides in Blood by UPLC-HRMS

YANG Yang, ZHANG Xiao-guang, YU Feng，et al .

Affiliations

YANG Yang, ZHANG Xiao-guang, YU Feng，et al .: 1. Forensic Identification Center of Hebei Medical University, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 2. Academy of Medicine and Health of Hebei Medical University, Core Facility of Hebei Medical University, Shijiazhuang 050017, China; 3. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China; 4. Institute of Forensic Science, Shijiazhuang Public Security Bureau, Shijiazhuang 050000, China

DOI: https://doi.org/10.12116/j.issn.1004-5619.2018.06.003
Journal volume & issue: Vol. 34, no. 6
pp. 590 – 594，600

Abstract

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Objective To develop a method to screen and quantify 10 common herbicides （paraquat, diquat, glyphosate, glufosinate, cyanazine, atrazine, metazachlor, acetochlor, chlorsulfuron, and metsulfuron） in blood. Methods With acetonitrile-water solution [V（acetonitrile）∶V（water）=3∶1] as protein precipitant, 10 common herbicides in blood were detected using ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）. Results All the 10 herbicides had good linearity in their linear range （coefficient of determination R2≥0.993）, with the recovery rates 67.4%-111.9%, the relative standard deviations 1.5%-10.8%, the accuracies 85.1%-106.1%, intra-day precisions 2.7%-13.5%, and inter-day precisions 3.3%-13.3%. Conclusion This method is easy to operate with high recovery rates. It enables rapid and accurate qualitative screening and quantitative analysis of various herbicides in blood simultaneously.

forensic toxicology; herbicide; ultra-high performance liquid chromatography-high resolution mass spectrometry; protein precipitation; biological samples

Published in Fayixue Zazhi

ISSN: 1004-5619 (Print)
Publisher: Editorial Office of Journal of Forensic Medicine
Country of publisher: China
LCC subjects: Medicine
Website: http://www.fyxzz.cn/EN/1004-5619/home.shtml

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