In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset; Conor C. Horgan; James P. K. Armstrong; Mads S. Bergholt; Vincenzo Torraca; Qu Chen; Timothy J. Keane; Laurence Bugeon; Margaret J. Dallman; Serge Mostowy; Molly M. Stevens

doi:10.1038/s41467-020-19827-1

Nature Communications (Dec 2020)

In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset,
Conor C. Horgan,
James P. K. Armstrong,
Mads S. Bergholt,
Vincenzo Torraca,
Qu Chen,
Timothy J. Keane,
Laurence Bugeon,
Margaret J. Dallman,
Serge Mostowy,
Molly M. Stevens

Affiliations

Håkon Høgset: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
Conor C. Horgan: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
James P. K. Armstrong: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
Mads S. Bergholt: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
Vincenzo Torraca: Department of Infection Biology, London School of Hygiene & Tropical Medicine
Qu Chen: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
Timothy J. Keane: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London
Laurence Bugeon: Department of Life Sciences, Imperial College London
Margaret J. Dallman: Department of Life Sciences, Imperial College London
Serge Mostowy: Department of Infection Biology, London School of Hygiene & Tropical Medicine
Molly M. Stevens: Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London

DOI: https://doi.org/10.1038/s41467-020-19827-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Raman spectroscopic imaging (RSI) can provide information on the chemical composition of a sample, but application to living organisms has lacked sufficient spatial resolution and signal strength. Here the authors apply confocal RSI to whole-mount zebrafish embryos to distinguish different infectious bacteria and to living zebrafish embryos to monitor the wound healing process.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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