Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats

Yiru Shao; Fangqing Zhou; Daikun He; Lin Zhang; Jie Shen

Biomedicine & Pharmacotherapy (Jan 2019)

Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats

Yiru Shao,
Fangqing Zhou,
Daikun He,
Lin Zhang,
Jie Shen

Affiliations

Yiru Shao: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Research Center for Chemical Injury, Emergency and Critical Care of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China
Fangqing Zhou: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Research Center for Chemical Injury, Emergency and Critical Care of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China
Daikun He: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Research Center for Chemical Injury, Emergency and Critical Care of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China
Lin Zhang: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Research Center for Chemical Injury, Emergency and Critical Care of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China
Jie Shen: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Center of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Medical Research Center for Chemical Injury, Emergency and Critical Care of Chemical Injury, Jinshan Hospital, Fudan University, Shanghai 201508, China; Corresponding author at: Center of Emergency & Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai 201508, China.

Journal volume & issue: Vol. 109
pp. 1233 – 1239

Abstract

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Phosgene exposure may result in acute lung injury (ALI) with high mortality. Emerging evidence suggests that mesenchymal stem cells (MSCs) have a therapeutic potential against ALI. CXC chemokine receptor 7 (CXCR7) has been identified as a receptor of stromal-cell-derived factor 1 (SDF1) involved in MSC migration and may be an important mediator of the therapeutic effects of MSCs on ALI. In our study, we initially constructed a lentiviral vector overexpressing CXCR7 and then successfully transduced it into rat bone marrow-derived MSCs (resulting in MSCs-CXCR7). We found that ALI and the wet-to-dry ratio significantly decreased in the phosgene-exposed rats after administration of MSCs-CXCR7 or MSCs-GFP. Indeed, treatment with MSCs-CXCR7 caused further improvement. Moreover, injection of MSCs-CXCR7 significantly facilitated MSC homing to injured lung tissue. Meanwhile, overexpression of CXCR7 promoted differentiation of MSCs into type II alveolar epithelial (AT II) cells and enhanced the ability of MSCs to modulate the inflammatory response in phosgene-induced ALI. Taken together, our findings suggest that CXCR7-overexpressing MSCs may markedly facilitate treatment of phosgene-induced ALI (P-ALI) in rats.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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