RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA: EFFECT ON IMMUNE RESPONSE IN MICE

Abstract

Aim. Study the effect of recombinant antigens of P. aeruginosa on key effectors of the immune system. Materials and methods. Mice were immunized intraperiotoneally with 25 jig of OprF and 50 jig of anatoxin sorbed on aluminium hydroxide gel with a 2 week interval. 7 days after the last immunization spleen lymphocyte subpopulation structure was evaluated by flow cytometry. Cytokine levels in mice sera were studied after a single immunization with recombinant OprF and anatoxin at 4, 8, 24 hours and 14 days by flow cytometry using FlowCytomix Mouse Thl/Th2 10 plex. Results. OprF recombinant antigens and anatoxin affect molecular-cell mechanisms of immune response resulting in alteration of expression of differentiating and activating molecules as well as synthesis of Thl/Th2/Thl7/Th21/Th22 cytokines in mice that are necessary for effective presentation of the antigen. Conclusion. Complex of recombinant OprF and anatoxin facilitated formation of complete immune response against pseudomonas.

Keywords

• pseudomonas aeruginosa
• pseudomonas aeruginosa
• outer membrane protein f
• anatoxin
• recombinant protein
• immune phenotype
• cytokines