Simplified ChIP-exo assays

Matthew J. Rossi; William K. M. Lai; B. Franklin Pugh

doi:10.1038/s41467-018-05265-7

Nature Communications (Jul 2018)

Simplified ChIP-exo assays

Matthew J. Rossi,
William K. M. Lai,
B. Franklin Pugh

Affiliations

Matthew J. Rossi: Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University
William K. M. Lai: Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University
B. Franklin Pugh: Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University

DOI: https://doi.org/10.1038/s41467-018-05265-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

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While ChIP-exo is low noise and highly informative regarding genome-wide binding proteins, libraries are difficult to construct. Here the authors present a simplified ChIP-exo method for high-resolution detection of interactions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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