FEATURES OF THE INFLUENCE ON ALLOCHTHONOUS MICROORGANISMS OF SPECIFIC BIOLOGICALLY ACTIVE COMPONENTS OF PACKAGED MINERAL WATERS

Abstract

Nowadays the issue of safety of bottled natural mineral water receives a lot of attention, as it’s consumption increases every year. Like any other product used by humans, it must be safe. Research from previous years confirms the importance of constant monitoring of the sanitary and microbiological safety of packaged mineral waters. The purpose of the work: to assess the influence of specific biologically active components (metasilicic and orthoboric acids) on the sanitary condition of packaged mineral waters (saturated and unsaturated), which was sold in Ukraine from 2017 to 2021. Materials and methods. Sanitary-indicative microorganisms in the studied mineral water were detected in several stages. During the researching were used methods withsolid and liquid nutrient media were used. Liquid glucose-peptone medium, Endo’s medium, oxidase test and microscopy were used to determine the number of coliform bacteria (CB). The total microbial count was determined on nutrient agar at 37°C for 24 hours. To determine Pseudomonas aeruginosa, a liquid glucose-peptone medium (GPM) was used as an accumulation medium, followed by cultivation on CPC agar, at 37°C for 24-48 hours, King-A medium, and nutrient agar at 42°C and 4°С, oxidase test and microscopy. Hugh-Leifson medium was used to determine oxidation and fermentation. The results. A regularity was determined in the number and degree of contamination of MW samples depending on the type and concentration of a specific biologically active component. Metasilicic and orthoboric acids have a unidirectional antibacterial effect, but in turn, the effect on sanitary indicator microorganisms of orthoboric acid is more pronounced. Carbon dioxide and autochthonous microbiota of the most natural MW act as factors enhancing the antibacterial action. Conclusions. It was determined that the studied specific biologically active components of natural packaged mineral waters are able to show antibacterial properties by enhancing the effects of each other.

Keywords

• packaged natural mineral waters,carbon dioxide,metasilicic acid,orthoboric acid,allochthonous microorganisms.