PLANT TRANSFORMATION: ADVANCES AND PERSPECTIVES

Abstract

Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation), screening (reporter gene possibilities and performance) and transformant selection (ipt selective marker) are discussed. Transgene expression and mechanisms underlying (trans)gene inactivation are presented. Practical applications of genetically modified plants, field tests and commercial transgenic crops worldwide and in Brazil are listed, as well as the main traits and species modified. Potential uses of transgenic plants for animal compound production, biological remediation and synthetic polymer assembly are also shown.

Keywords

• plant transformation
• Agrobiolistics
• electroporation
• reporter genes
• transgenic crops