Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress

Abstract

Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the callose synthase genes is limited. In this study, we analyzed the callose synthase gene family of soybean at the genome-wide scale. We also studied the genes positions, gene structure, evolutionary relations, miRNAs target sites, and expression of CalS genes. Resultantly 24 CalS genes were found in soybean, with diverse chromosomal locations, cis-acting elements, conserved motifs, and gene structures. Further, GmCalS genes were divided into four phylogenetic classes. The evolutionary classification of CalSs was supported by the motif and gene structure analyses. Phytohormones, abiotic stresses, and growth-responsive elements were identified in the promoter of GmCalSs. In addition, the GmCalS genes higher expression in roots, leaves, flowers, and nodules tissues provided their significance in development. Furthermore, the higher expression of GmCalS17 and GmCalS19 genes in response to salt stress indicated their importance against salt stress. These findings will be helpful for further investigation of the CalS genes in other crops.

Keywords

• Callose Synthase
• Expression
• Growth
• Regulation
• qRT-PCR
• Salt