Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation

Abstract

Objective: The main purpose of the current study was to determine the effect of nano-capsules containing risedronate on calvarial bone formation in rabbit. Materials and Methods: Fifteen adult rabbits were allocated to the study. Four holes were created in the calvarial bone. Holes 1-4 were filled as described below: hole 1 was right unfilled and kept as control; hole 2 was filled with nano bone; hole 3 was filled using an autogenous bone; and hole 4 was filled with a mixture of nano-capsules containing risedronate. At 4, 8 and 12 weeks after surgery, blood samples were obtained and red blood cell, white blood cell, hemoglobin, hematocrit, mean cell volume, mean cell HGB and platelet counts were determined. Then animals were scarified and bone density was determined using radiography images. Results: Bone formation in nano risedronate + autograft and autograft were 0.31 ± 0.03 and 0.25 ± 0.02, respectively, while in nano risedronate and control groups were 0.11 ± 0.01 and 0.08 ± 0.02, respectively (P 0.05). Conclusion: These results suggest nano risedronate + autograft has positive effects on calvarial bone defects healing in rabbit.

Keywords

• Nano-capsules
• Risedronate
• Calvarial healing
• Radiography
• Rabbit