Acta Biológica Colombiana (Jul 2005)

Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a

  • Carlos Rivera R.,
  • Ángela Zapata A.,
  • Gabriel Pinilla,
  • Jhon Donato,
  • Beatriz Chaparro,
  • Paola Jiménez

Journal volume & issue
Vol. 10, no. 2
pp. 95 – 103

Abstract

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In four mountain aquatic ecosystems of altiplano Cundiboyacense, Colombia, we compared through analysis regression data set of chlorophyll-a obtained with spectoropho-tometric (tricromatic formula) and fluorometric (Welschmeyer) methods. The analysis showed that the spectrophotometric method overestimated the chlorophyll-a concen-tration, but it could be used with precaution in the lower trophic environments. An equation that relates the two chlorophyll-a measures was obteined.

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