The Effects of Pre-Storage Leukoreduction on the Conservation of Bovine Whole Blood in Plastic Bags
Brena Peleja Vinholte,
Rejane dos Santos Sousa,
Francisco Flávio Vieira Assis,
Osvaldo Gato Nunes Neto,
Juliana Machado Portela,
Gilson Andrey Siqueira Pinto,
Enrico Lippi Ortolani,
Fernando José Benesi,
Raimundo Alves Barrêto Júnior,
Antonio Humberto Hamad Minervino
Affiliations
Brena Peleja Vinholte
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Rejane dos Santos Sousa
Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, Butantã, São Paulo 05508-270, SP, Brazil
Francisco Flávio Vieira Assis
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Osvaldo Gato Nunes Neto
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Juliana Machado Portela
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Gilson Andrey Siqueira Pinto
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Enrico Lippi Ortolani
Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, Butantã, São Paulo 05508-270, SP, Brazil
Fernando José Benesi
Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, Butantã, São Paulo 05508-270, SP, Brazil
Raimundo Alves Barrêto Júnior
Department of Animal Science, Federal Rural University of the Semiarid Region, UFERSA, Av. Francisco Mota, 572-Bairro Costa e Silva, Mossoró 59625-900, RN, Brazil
Antonio Humberto Hamad Minervino
Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Rua Vera Paz, s/n Bairro Salé, Santarém 68040-255, PA, Brazil
Leukoreduction (LR) is a technique that consists of reducing the number of leukocytes in whole blood or blood components that can contribute to decreasing storage lesions and the occurrence of post-transfusion complications. We propose that using a blood bag with pre-storage leukocyte filtration is sufficient for blood conservation under field conditions. Ten healthy Nelore cows were used. Whole blood was sampled from each animal and stored at 2 to 6 °C in CPD/SAG-M (citrate phosphate dextrose bag with a saline, adenine, glucose, mannitol satellite bag) triple bags (Control) and in CPD/SAG-M quadruple bags with a leukocyte filter (Filter). At baseline and after 7, 14, 21, 28, 35, and 42 days (D0, D7, D14, D21, D28, D35, and D42, respectively), complete hematological, blood gas, and biochemical evaluations were determined. The filtered bag removed 99.3% of white blood cells from cattle blood, and the entire filtration process was performed in the field. There was a reduction in the number of red blood cells (RBCs) in both groups from D14 onward, with a decrease of 19.7% and 17.1% at D42 for the Control and Filter bags, respectively. The hemoglobin (Hb) concentration had variation in both groups. Potassium, pO2, pCO2, and sO2 increased, and sodium, bicarbonate, and pH decreased during storage. The filtered bag was efficient in removing white cells from cattle whole blood and could be used under field conditions. Blood stored after LR showed differences (p 2, higher sO2). Further experimental studies are required to prove that blood without white cells results in a decrease in transfusion reactions in cattle.
