Scientific Reports (Jun 2022)

RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation

  • Aude Guénolé,
  • Fabien Velilla,
  • Aymeric Chartier,
  • April Rich,
  • Anne-Ruxandra Carvunis,
  • Claude Sardet,
  • Martine Simonelig,
  • Bijan Sobhian

DOI
https://doi.org/10.1038/s41598-022-13309-8
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 17

Abstract

Read online

Abstract Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner. Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to repress translation of a target mRNA. We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism.