Nature Communications (Jan 2019)

Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

  • Vivek K. Mutalik,
  • Pavel S. Novichkov,
  • Morgan N. Price,
  • Trenton K. Owens,
  • Mark Callaghan,
  • Sean Carim,
  • Adam M. Deutschbauer,
  • Adam P. Arkin

DOI
https://doi.org/10.1038/s41467-018-08177-8
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 14

Abstract

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Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.