Nature Communications (Jan 2019)
Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
Abstract
Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.