Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Abstract

Summary: Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozygous APOE knockout (APOE-/-) iPSCs and further validate the deficiency of apoE in iPSC-derived cerebral organoids. APOE-/- cerebral organoids can serve as a useful tool to study apoE functions and apoE-related pathogenic mechanisms in AD.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).

Keywords

• CRISPR
• Neuroscience
• Stem Cells
• Organoids