Enhanced UV-B photoprotection activity of carotenoids from the novel Arthrobacter sp. strain LAPM80 isolated from King George Island, Antarctica
Beatriz Vivian Paredes Contreras,
Alane Beatriz Vermelho,
Livia Casanova,
Claudia de Alencar Santos Lage,
Caren Leite Spindola Vilela,
Veronica da Silva Cardoso,
Luis William Pacheco Arge,
Janine Simas Cardoso-Rurr,
Sulamita Santos Correa,
Felipe Raposo Passos De Mansoldo,
Maria Cristina Pinheiro Pereira Reis-Mansur,
Eikon Alves da Silva,
Júnia Schultz,
Alexandre Soares Rosado
Affiliations
Beatriz Vivian Paredes Contreras
Laboratory of Molecular Microbial Ecology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Alane Beatriz Vermelho
BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Livia Casanova
BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Claudia de Alencar Santos Lage
Laboratory of Radiations in Biology, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Caren Leite Spindola Vilela
Laboratory of Molecular Microbial Ecology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Veronica da Silva Cardoso
BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Luis William Pacheco Arge
Laboratory of Molecular Genetics and Plant Biotechnology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Janine Simas Cardoso-Rurr
Laboratory of Radiations in Biology, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Sulamita Santos Correa
Laboratory of Molecular Microbial Ecology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Felipe Raposo Passos De Mansoldo
BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Maria Cristina Pinheiro Pereira Reis-Mansur
BioInovar Laboratory, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Eikon Alves da Silva
Laboratory of Molecular Microbial Ecology, Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Júnia Schultz
Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Makkah, 23955, Saudi Arabia
Alexandre Soares Rosado
Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Makkah, 23955, Saudi Arabia; Corresponding author.
Antarctica's harsh environmental conditions, characterized by high levels of ultraviolet (UV) radiation, pose challenges for microorganisms. To survive in these extreme cold regions with heightened UV exposure, microorganisms employ various adaptive strategies, including photoprotective carotenoid synthesis. Carotenoids are garnering attention in the skin health industry because of their UV photoprotection potential, given the direct relationship between UV exposure and skin burns, and cancer. Also, there is a growing demand for natural and environmentally friendly photoprotectors, such as microbial-based products, in opposition to synthetic photoprotective agents with known adverse effects.In this study, we assessed the carotenoid-producing abilities of Actinomycetota strains from Antarctic Peninsula soils and the photoprotective carotenoid action on UV irradiation resistance. Among 20 evaluated strains, one exhibited significant carotenoid production and it was identified through genomic analysis as a likely novel Arthrobacter sp. strain, LAPM80. This strain's genome revealed the presence of genes coding for the biosynthesis of decaprenoxanthin C50 carotenoid. The LAPM80 strain exhibited enhanced resistance against UV-B irradiation, correlating with increased total carotenoid production in its stationary growth phase. Chemical characterization of the carotenoid extract identified major components as C50 carotenoids, probably decaprenoxanthin and/or sarcinaxanthin. Scanning electron microscopy revealed minimal surface changes in bacteria during carotenoid-rich phase after UV-B irradiation exposure.These findings highlight the likely ability of LAPM80 strain's C50 carotenoids to improve UV-B iiradiation resistance, indicating their potential for developing natural photoprotective compounds for the dermo-cosmetic industry.
