Nature Communications (Jun 2021)

Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A

  • Yuqin Wang,
  • Xiaoyu Guan,
  • Shanyu Zhang,
  • Yao Liu,
  • Sha Wang,
  • Pingping Fan,
  • Xiaoyu Du,
  • Shuanghong Yan,
  • Panke Zhang,
  • Hong-Yuan Chen,
  • Wenfei Li,
  • Daoqiang Zhang,
  • Shuo Huang

DOI
https://doi.org/10.1038/s41467-021-23764-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Nanopores have been used for direct observation of RNA structure in native environments but have limited RNA differentiation capabilities. Here, the authors report on the use of Mycobacterium smegmatis porin A nanopores for the trapping and translocation identification of microRNA, siRNA, tRNA and ribosomal RNA.