Study on Antibacterial and Quorum-Sensing Inhibition Activities of <i>Cinnamomum camphora</i> Leaf Essential Oil

Wenting Wang; Dongxiang Li; Xiaoqin Huang; Huixiang Yang; Ziwen Qiu; Liting Zou; Qin Liang; Yu Shi; Yingxiang Wu; Shaohua Wu; Chao Yang; Yongyu Li

doi:10.3390/molecules24203792

Molecules (Oct 2019)

Study on Antibacterial and Quorum-Sensing Inhibition Activities of <i>Cinnamomum camphora</i> Leaf Essential Oil

Wenting Wang,
Dongxiang Li,
Xiaoqin Huang,
Huixiang Yang,
Ziwen Qiu,
Liting Zou,
Qin Liang,
Yu Shi,
Yingxiang Wu,
Shaohua Wu,
Chao Yang,
Yongyu Li

Affiliations

Wenting Wang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Dongxiang Li: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Xiaoqin Huang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Huixiang Yang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Ziwen Qiu: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Liting Zou: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Qin Liang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Yu Shi: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Yingxiang Wu: Qingyuan Agricultural Science and Technology Extension Service Center, Qingyuan 511518, China
Shaohua Wu: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Chao Yang: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Yongyu Li: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China

DOI: https://doi.org/10.3390/molecules24203792
Journal volume & issue: Vol. 24, no. 20
p. 3792

Abstract

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Many essential oils (EOs) regulate the quorum-sensing (QS) system of pathogens and inhibit the virulence expression. Interference with QS can potentially reduce bacterial multidrug resistance and aid the biological control of bacterial disease. In the present work, the antibacterial and anti-QS activities of Cinnamomum camphora leaf EO were investigated. A total of 23 chemical components with relative levels ≥0.11%, including a large number of terpene compounds, were identified in C. camphora leaf EO by gas chromatography−mass spectrometry (GC-MS). The principal component was linalool, followed by eucalyptol, with relative levels of 51.57% and 22.07%, respectively. The minimum inhibitory concentration (MIC) and antibacterial activity of C. camphora EO were examined, and P. aeruginosa and E. coli ATCC25922 showed the highest and lowest sensitivity to C. camphora EO, respectively. Tests of QS inhibitory activity revealed that C. camphora EO significantly decreased the production of violacein and biofilm biomass in C. violaceum, with the maximum inhibition rates of 63% and 77.64%, respectively, and inhibited the biofilm formation and swarming movement, independent of affecting the growth of C. violaceum. Addition of C. camphora EO also resulted in downregulation of the expression of the acyl-homoserine lactones (AHL) synthesis gene (cviI) and transcription regulator (cviR), and inhibited the expression of QS-regulated virulence genes, including vioA, vioB, vioC, vioD, vioE, lasA, lasB, pilE3, and hmsHNFR. Collectively, the prominent antibacterial activity and anti-QS activities clearly support that C. camphora EO acts as a potential antibacterial agent and QS inhibitor in the prevention of bacterial contamination.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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