Exploring the genetic progression of MDR1 in Plasmodium falciparum: A decade of multi-regional genetic analysis (2014–2024)

Abstract

Summary: Background: The genetic progression of the MDR1 gene in Plasmodium falciparum, a key factor in drug resistance, presents significant challenges for malaria control. This study aims to elucidate the genetic diversity and evolutionary dynamics of P. falciparum, particularly focusing on the MDR1 gene across multi-regional populations. To analyze the genetic diversity of P. falciparum MDR1 gene across various multi-regional populations between 2014 and 2024, assessing allelic richness, genetic distances, and evolutionary patterns. Methods: We conducted an extensive genetic analysis using methods such as Analysis of Molecular Variance (AMOVA), pairwise population matrices of Nei unbiased genetic distance and identity, PhiPT and Phi'PT values, and Principal Coordinates Analysis (PCoA). The study covered diverse P. falciparum populations from India, Nigeria, Ethiopia, Honduras, China, and Cameroon. Findings: Our findings reveal significant genetic heterogeneity in the MDR1 gene. Populations like India: Odisha (2014) exhibited high allelic richness, indicating diverse drug resistance profiles. Notable genetic divergence was observed, especially between India (2016) and Nigeria (2020), suggesting different evolutionary trajectories in drug resistance. The PCoA analysis highlighted the multi-dimensional genetic variation, reflecting the complex interplay of factors influencing drug resistance in P. falciparum. Interpretation: The comprehensive analysis of P. falciparum's MDR1 gene provides crucial insights into the multi-regional patterns of drug resistance. This knowledge is essential for developing effective malaria control measures and adapting treatment strategies to the evolving genetic diversity of the parasite.

Keywords

• MDR1 gene
• P. falciparum
• Genetic diversity
• Evolutionary dynamics
• Malaria