Revista Brasileira de Obesidade, Nutrição e Emagrecimento (Jan 2023)

Gene expression of MTOR, MURF-1 and MAFBX in wistar rats supplemented with whey proteins

  • Marcos Roberto Campos de Macêdo,
  • Antonio Coppi Navarro,
  • Raphael Furtado Marques,
  • Melaine Mont´Alverne Lawall Silva,
  • Flavia Castello Branco Vidal,
  • Alanna Joselle Santiago Silva,
  • Carlos Eduardo Neves Amorim,
  • Francisco Navarro

Journal volume & issue
Vol. 16, no. 104
pp. 907 – 917

Abstract

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To verify the effect of supplementation of different doses of Whey Proteins for 12 weeks on the gene expression of MTOR, MURF-1, MAFBX and on the alteration of the cellular diameter of the gastrocnemius muscle. Sample: 38 Wistar rats and supplemented for 12 weeks. The white portion of the right gastrocnemius muscle was embedded in Rna Latter solution and stored at -80 ° C and subjected to real-time polymerase chain reaction analysis. The left gastrocnemius muscle was intended for histological analysis. Gene expression of MTOR mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than in the control group, in the order of 100%, 97%, 96%, respectively. Likewise, the gene expression of MURF-1 mRNA had lower statistical significance in the groups of Whey 2, Whey 4, Whey 6 than the control group, in the order of 65%, 75%, 84%, respectively. In line, the gene expression MAFBX mRNA had lower statistical significance in the groups Whey 2, Whey 4, Whey 6 in relation to the control group, in the order of 99%, 97%, 99%, respectively. There was no statistical difference in the cellular area of the gastrocnemius muscle between the groups. Therefore, supplementation of Whey Proteins at doses of 2, 4, 6 g / kg / day for 12 weeks in sedentary animals was not able to increase the gene expression of MTOR mRNA, however, it was able to reduce the gene expression of MURF -1 mRNA, MAFBX mRNA.

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