Nature Communications (Aug 2020)
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
- Lucy I. Crouch,
- Marcelo V. Liberato,
- Paulina A. Urbanowicz,
- Arnaud Baslé,
- Christopher A. Lamb,
- Christopher J. Stewart,
- Katie Cooke,
- Mary Doona,
- Stephanie Needham,
- Richard R. Brady,
- Janet E. Berrington,
- Katarina Madunic,
- Manfred Wuhrer,
- Peter Chater,
- Jeffery P. Pearson,
- Robert Glowacki,
- Eric C. Martens,
- Fuming Zhang,
- Robert J. Linhardt,
- Daniel I. R. Spencer,
- David N. Bolam
Affiliations
- Lucy I. Crouch
- Biosciences Institute, Faculty of Medical Sciences, Newcastle University
- Marcelo V. Liberato
- Universidade de Sorocaba, Programa de Processos Tecnológicos e Ambientais
- Paulina A. Urbanowicz
- Ludger Ltd, Culham Science Centre
- Arnaud Baslé
- Biosciences Institute, Faculty of Medical Sciences, Newcastle University
- Christopher A. Lamb
- Department of Gastroenterology, Newcastle upon Tyne Hospitals NHS Foundation Trust
- Christopher J. Stewart
- Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University
- Katie Cooke
- Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University
- Mary Doona
- Department of Gastroenterology, Newcastle upon Tyne Hospitals NHS Foundation Trust
- Stephanie Needham
- Department of Histopathology, Newcastle upon Tyne Hospitals NHS Foundation Trust
- Richard R. Brady
- Department of Colorectal Surgery, Newcastle upon Tyne Hospitals NHS Foundation Trust
- Janet E. Berrington
- Newcastle Neonatal Service, Royal Victoria Infirmary
- Katarina Madunic
- Centre for Proteomics and Metabolomics, Leiden University Medical Centre
- Manfred Wuhrer
- Centre for Proteomics and Metabolomics, Leiden University Medical Centre
- Peter Chater
- Biosciences Institute, Faculty of Medical Sciences, Newcastle University
- Jeffery P. Pearson
- Biosciences Institute, Faculty of Medical Sciences, Newcastle University
- Robert Glowacki
- Department of Microbiology and Immunology, University of Michigan Medical School
- Eric C. Martens
- Department of Microbiology and Immunology, University of Michigan Medical School
- Fuming Zhang
- Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
- Robert J. Linhardt
- Department of Chemistry and Chemical Biology, Centre for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
- Daniel I. R. Spencer
- Ludger Ltd, Culham Science Centre
- David N. Bolam
- Biosciences Institute, Faculty of Medical Sciences, Newcastle University
- DOI
- https://doi.org/10.1038/s41467-020-17847-5
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Epithelial cells that line the gut secrete complex glycoproteins that form a mucus layer to protect the gut wall from enteric pathogens. Here, the authors provide a comprehensive characterisation of endo-acting glycoside hydrolases expressed by mucin-degrading members of the microbiome that are able to cleave the O-glycan chains of a range of different animal and human mucins.
