The Effect of Energy Drink on Histomorphological Changes in Skeletal Muscles of Wistar Albino Rats

Abstract

Objective: To observe the histomorphological alterations induced by consumption of energy drinks on skeletal muscles of Albino rats. Study Design:An experimental study. Place and Duration of Study: The study was conducted by the Department of Anatomy in collaboration with the animal house of Liaquat national hospital and medical college (LNH&MC), Karachi from November 2 to December 1 2020. Materials and Methods: For the study, 30 adult male Wistar Albino rats weighing between 140 and 200 grammes were allocated evenly into 3 groups. Group A served as control, kept on a regular laboratory diet. Group B served as a low dose treated group receiving energy drink at a dose of 7.5ml/day while group C received a high dose of drink i-e 15ml/day via gastric tube. All the animals were sacrificed following completion of the experimental period and were subjected to microscopic examination for histo-morphological study. Data was analyzed by using SPSS version 25. Results: The Hematoxylin and Eosin (H&E) stained sections revealed significant structural and parenchymal damage in skeletal muscle tissue. The regular parallel arrangement of skeletal muscle fibers was lost with the disappearance of nuclei. Dilated and congested blood vessels were observed in the treated tissues. Infiltration of mononuclear cells was also observed suggesting the inflammatory changes in the tissues of animals treated with caffeinated beverages in the present study. Conclusion: The consumption of energy drinks produces a significant histo-morphological alteration in the skeletal muscles of Wistar Albino rats

Keywords

• congested blood vessels
• energy drink
• loss of nuclei
• mononuclear cells
• skeletal muscles.