Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Yunqiao Yang; Kaiyuan Hao; Mingsheng Jiang; Fareed Uddin Memon; Lei Guo; Geyin Zhang; Tian Liu; Xianshi Wu; Hongbin Si

doi:10.3390/genes12071003

Genes (Jun 2021)

Transcriptomic Analysis of Drug-Resistance Acinetobacter baumannii under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing

Yunqiao Yang,
Kaiyuan Hao,
Mingsheng Jiang,
Fareed Uddin Memon,
Lei Guo,
Geyin Zhang,
Tian Liu,
Xianshi Wu,
Hongbin Si

Affiliations

Yunqiao Yang: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Kaiyuan Hao: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Mingsheng Jiang: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Fareed Uddin Memon: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Lei Guo: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225012, China
Geyin Zhang: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Tian Liu: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Xianshi Wu: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China
Hongbin Si: College of Animal Sciences and Technology, Guangxi University, Nanning 530004, China

DOI: https://doi.org/10.3390/genes12071003
Journal volume & issue: Vol. 12, no. 7
p. 1003

Abstract

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Litsea cubeba L. essential oil (LCEO) can affect the growth of drug-resistance bacteria. However, research on stress response of drug-resistant A. baumannii under sub-lethal LCEO concentrations had been limited so far. Therefore, transcriptomic analysis of A. baumannii under 1/2 minimum inhibitory concentration (MIC, 0.54 mg/mL) of LCEO was performed. Results of transcriptomic analysis showed that 320/352 genes were significantly up/down-regulated, respectively, in LCEO-treated A. baumannii. Both up and down-regulated genes were significantly enriched in three GO terms (oxidation-reduction process; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors), which indicated that the redox state of A. baumannii was significantly affected by LCEO. LCEO may also inhibit aerobic respiration, synthesis of ketone bodies and the metabolism of some amino acids while, meanwhile, promoting fatty acid degradation of A. baumannii according to Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. The permeability and the stress of cell membrane of A. baumannii were significantly affected by LCEO. After crystal violet dyeing, the biofilm formation of A. baumannii was promoted/inhibited by extremely low/relatively high concentration of LCEO, respectively. LCEO and chloramphenicol have synergistic growth inhibitory effect against A. baumannii according to the Fractional Inhibitory Concentration Index (FICI) value = 0.375. Our results indicate that the growth of A. baumannii was inhibited by LCEO, and give insights into the stress response of A. baumannii under sub-lethal concentrations of LCEO. These results provided evidence that A. baumannii was inhibited by LCEO, and expanded knowledges of stress response of A. baumannii under sub-lethal concentration of LCEO.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

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