The metabolism of 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid into cholic: an enzyme assay using homogenates of human liver

R F Hanson; H L Sharp; G C Williams

Journal of Lipid Research (May 1976)

The metabolism of 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid into cholic: an enzyme assay using homogenates of human liver

R F Hanson,
H L Sharp,
G C Williams

Affiliations

R F Hanson: Gastroenterology Units, Department of Internal Medicine and Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455
H L Sharp: Gastroenterology Units, Department of Internal Medicine and Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455
G C Williams: Gastroenterology Units, Department of Internal Medicine and Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455

Journal volume & issue: Vol. 17, no. 3
pp. 294 – 297

Abstract

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An enzyme assay was developed to measure the conversion of the bile acid precursor, 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 ± 0.5 (± 1 SD) pmoles of cholic acid formed/mg liver/minute in twelve normal liver biopsies. This assay system can be used to determine if the syndrome of neonatal cholestasis associated with a metabolic block in the conversion of THCA into cholic acid is transmitted as a genetic trait.

bile acid synthesis

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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