Effects of a fully enclosed hollow-fiber centrifugal ultrafiltration technique for laboratory biosafety improvement

Abstract

Laboratory biosafety has become a core focus in biological analysis, owing to the frequent occurrence of laboratory-acquired infections caused by the leakage of pathogenic microorganisms. For this purpose, the authors developed a safe pretreatment device combining a sealing technique with a direct injection technique. In this study, several bacteria and viruses were used to validate the filtration effect of the invention. Data show that the new device can completely filter bacteria and that the filtration rates for hepatitis B virus and hepatitis C virus reached 94% and 96%, respectively. The results show that the new preparation device can effectively block these pathogens and can improve biological safety and provide powerful protection for technicians.

Keywords

• bacterium
• centrifuge tube
• fully enclosed hollow fiber centrifugal ultrafiltration
• laboratory-acquired infections
• laboratory biosafety
• virus